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Extraction of DNA from cells [videorecording]

by Films for the Humanities & Sciences (Firm).
Type: materialTypeLabelVisual MaterialSeries: DNA in practice .Publisher: Princeton, NJ Films for the Humanities & Sciences, c1993Description: 1 videocassette (15 min.) : sd., col. ; 1/2 in.Subject(s): DNA -- Separation | Genetic engineering | Human genetics | GeneticsSummary: Shows the process of DNA extraction: grinding liver tissue using liquid nitrogen to prevent degradation of cellular contents, adding a lysis buffer, rupturing the cell membrane to liberate cellular contents, and separating the DNA from other cellular components such as protein. After several further steps, the DNA is lifted out, dissolved in a small volume of distilled water, and the concentration of DNA in the solution estimated by means of a spectrophotometer. Also shows how restriction enzymes are used to cut the DNA into shorter pieces.
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FFH 3993

Shows the process of DNA extraction: grinding liver tissue using liquid nitrogen to prevent degradation of cellular contents, adding a lysis buffer, rupturing the cell membrane to liberate cellular contents, and separating the DNA from other cellular components such as protein. After several further steps, the DNA is lifted out, dissolved in a small volume of distilled water, and the concentration of DNA in the solution estimated by means of a spectrophotometer. Also shows how restriction enzymes are used to cut the DNA into shorter pieces.

VHS/NTSC

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